Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Direct Injection Method for Quantitation of Endogenous Leukotriene E4 in Human Urine by Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry with a Column-switching Technique
Naoya KISHINariyasu MANONaoki ASAKAWA
Author information
JOURNAL FREE ACCESS

2001 Volume 17 Issue 6 Pages 709-713

Details
Abstract

A new analytical method employing liquid chromatography/tandem mass spectrometry (LC/MS/MS) with a column-switching system was developed for quantitative determination of leukotriene E4 (LTE4) in human urine. A column-switching system using a trapping column, which concentrates the analyte and removes salts and other water-soluble contaminants, allowed direct injection of human urine. Because simultaneously eluted endogenous contaminants suppressed the ionization efficiency of LTE4, good liquid chromatographic separation was very important for establishing this method, notwithstanding the high selectivity of MS/MS. The calibration curve was linear over the range from 10 to 3000 pg/mL, and the method showed good accuracy and precision. This method should therefore be very useful for determination of LTE4 amounts in human urine in studies on leukotriene metabolism and the efficacy of antileukotriene drugs.

Content from these authors
© 2001 by The Japan Society for Analytical Chemistry
Previous article Next article
feedback
Top