2002 Volume 18 Issue 10 Pages 1117-1120
Capillary zone electrophoresis was employed for the determination of histamine using end-column amperometric detection with a carbon fiber microelectrode, at a constant potential. The optimum conditions of separation and detection were 10 mmol/L phosphate buffer, pH 5.6 for the buffer solution, 15 kV for the separation voltage, and 1.35 V (versus SCE) for the detection potential. The linear range was from 6.3 × 10-7 to 1.5 × 10-5 mol/L with the regression coefficient of 0.9997, and the detection limit was 4.0 × 10-7 mol/L (S/N = 3). The proposed method was successfully applied to the direct determination of histamine in the beer samples without any sample clean-up procedures.