The preparation and utilization of a novel composite silica-hemin nanoparticles (CSHNs) as mimetic peroxidase are reported in this article. Experimental results showed that the composite nanoparticles had unique advantages over free hemin molecules in good stability and highly catalytic activity. By employing these nanoparticles as biocatalyst, we developed a new spectrofluorometric method for the determination of trace level glucose. The calibration graph for glucose was linear over the range 4.0 × 10-7 mol/L - 7.0 × 10-5 mol/L, with a detection limit of 1.0 × 10-7 mol/L. The proposed method has been successfully applied to the determination of glucose in serum samples and offers the advantages of being rapid, stable, sensitive, and renewable.
2004 by The Japan Society for Analytical Chemistry