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Analytical Sciences
Vol. 22 (2006) No. 10 P 1291-1295

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http://doi.org/10.2116/analsci.22.1291

Original Papers

An inverted microscope was combined with a compact dye laser with a pulse width of < 190 ps and an intensified charge-coupled device (ICCD) camera with a minimum gate width of 200 ps. The resulting fluorescence lifetime imaging microscope, which has a temporal resolution of 340 ps, was used to measure the fluorescence lifetime of polymer microspherers. The results indicated a fluorescence lifetime of 0.9 ns. The present analytical instrument was also employed in an evaluation of biological cells after labeling them with SYTO 13, a fluorescent dye.

Copyright © 2006 by The Japan Society for Analytical Chemistry

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