Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Reviews
Properties of Monolithic Silica Columns for HPLC
Hiroshi KOBAYASHITohru IKEGAMIHiroshi KIMURATakeshi HARADaisuke TOKUDANobuo TANAKA
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2006 Volume 22 Issue 4 Pages 491-501

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Abstract

Monolithic silica columns and their use in high peak-capacity HPLC separations are reviewed. Monolithic silica columns can potentially provide higher overall performance than particle-packed columns based on the variable external porosity and variable through-pore size/skeleton size ratios. The high permeability of monolithic silica columns resulting from the high porosity is shown to be advantageous to generate large numbers of theoretical plates with long capillary columns. High permeability together with the high stability of the network structures of silica allows their use in high-speed separations required for a second-dimension column in two dimensional HPLC. Disadvantages of monolithic silica columns are also described.

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© 2006 by The Japan Society for Analytical Chemistry
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