Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Large-Volume Sample Sweeping with a High Theoretical Plate Number Using a Coupled-Capillary in Capillary Electrophoresis
Yen-Sheng CHANGChun-Min SHIH,Yen-Cheng LICheng-Huang LIN
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2006 Volume 22 Issue 4 Pages 557-561

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Abstract

A large-volume sample injection (> 5 µL) with an extremely high theoretical plate number (N > 107) was achieved when the sweeping-MEKC mode and a coupled-capillary (100 - 50 µm i.d.) were simultaneously used in a capillary electrophoresis (CE) separation. A low-cost and compact violet-LED (∼2 mW) was used as the fluorescence excitation source. As a result, the theoretical plate numbers of the detected peaks (two model compounds: naphthalene-2,3-dicarboxaldehyde derivatized-dopamine and -norepinephrine) were 1.0 × 107 and 7.4 × 106, respectively. The limits of detection (at S/N = 3) of these were determined to be 2.8 × 10-10 M (92 ppt) and 2.3 × 10-10 M (83 ppt), respectively.

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© 2006 by The Japan Society for Analytical Chemistry
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