Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Flow-Injection Determination of Thyroxine Using Immobilized Enzyme with Tris(2,2′-bipyridyl)ruthenium(III) Chemiluminescence Detection
Amir WASEEMMohammad YAQOOBAbdul NABI
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2006 Volume 22 Issue 8 Pages 1095-1098

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Abstract

A flow-injection method is reported for the determination of thyroxine based on its enhancement effect on the tris(2,2′-bipyridyl)ruthenium(III) chemiluminescence reaction in the presence of NADH using immobilized alcohol dehydrogenase purified from baker yeast. The limit of detection (3 σ blank) was 5.0 × 10-8 M with a sample throughput of 80 h-1. The calibration graph was linear over the range 0.5 - 10 × 10-7 M (r2 = 0.9988) with the relative standard deviation in the range 1.4 - 3.5% (n = 4). The effect of common excipients used in pharmaceutical preparations, some organic compounds and metal ions was studied. The method was applied to pharmaceutical thyroxine tablets, and the obtained results were not significantly different from the amount quoted.

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© 2006 by The Japan Society for Analytical Chemistry
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