Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Notes
Determination of Fructosyl Amino Acids and Fructosyl Peptides in Protease-digested Blood Sample by a Flow-Injection System with an Enzyme Reactor
Yoko NANJORyuzo HAYASHIToshio YAO
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2006 Volume 22 Issue 8 Pages 1139-1143

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Abstract

A flow-injection system with an enzyme reactor was proposed for the measurement of fructosyl amino acids and fructosyl peptides in protease-digested blood samples. A fructosyl-amino acid oxidase (FAOX-TE) and two fructosyl-peptide oxidases (FPOX-CE and FPOX-CET) were covalently immobilized onto an inert support. They were used as the enzyme reactor in a FIA system with a hydrogen peroxide electrode. In particular, the FPOX-CET reactor possessed high selectivity for the detection of fructosyl valine (FV) and fructosyl valyl histidine (FVH) and an excellent operational stability. The proposed FIA system with the FPOX-CET reactor responded linearly to the concentration of FV over the dynamic range of 7.8 × 10-6 to 5.8 × 10-4 M. The present method could be successfully applied to the assay of FV and FVH in the protease-digested blood samples.

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© 2006 by The Japan Society for Analytical Chemistry
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