Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
An Amperometric Glucose Biosensor Based on Titania Sol-gel/Prussian Blue Composite Film
Ruping LIANGJingli JIANGJianding QIU
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2008 Volume 24 Issue 11 Pages 1425-1430


An improved amperometric glucose biosensor was constructed by immobilizing glucose oxidase (GOD) in a titania sol-gel film, which was prepared by a vapor deposition method, on a Prussian Blue (PB)-modified electrode. The method combined the merits of immobilizing biomolecules in the titania sol-gel film by vapor deposition method and the synergic catalysis effects of PB and GOD molecules. Results showed that the fabricated titania sol-gel/PB membrane possessed high surface area, good mechanical stability, and good hydrophilicity, which provided a biocompatible microenvironment for maintaining the bioactivity of the immobilized enzyme and prevented the enzyme from leaking out of the film. Therefore, the present biosensor exhibited fast response time (10 s), high sensitivity (12.74 μA cm-2 mM-1), long-term operational stability, good suppression of interference, and a wide linear range from 0.02 to 15 mM with a low detection limit of 5 μM for the detection of glucose. In addition, this simple and controllable method could fabricate biosensors in batches with a very small amount of enzyme.

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© 2008 by The Japan Society for Analytical Chemistry
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