Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Simultaneous Determination of Venlafaxine and Its Main Active Metabolite O-Desmethyl Venlafaxine in Rat Plasma by LC-MS/MS
Gaurang R. SHAHBharat T. THAKERKiran R. SURATIMinu H. PARABIA
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2009 Volume 25 Issue 10 Pages 1207-1210

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Abstract

A simple, sensitive and selective liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and validated for the analysis of venlafaxine (VX) and its major active metabolite O-desmethyl venlafaxine (ODV) in rat plasma using carbamazepine as an internal standard (IS). The analytes from the biological matrix were extracted by liquid-liquid extraction using tert-butyl methyl ether (TBME). The HPLC separation of the analytes was performed on a water symmetry C18 (150 × 4.6 mm i.d., 5 μm) column, using a 10 mM ammonium formate:methanol (20:80 v/v) as mobile phase. The calibration curve concentration range was 10.10 to 8000.00 ng/mL for VX and ODV with coefficient of determination above 0.9970. The lower limit of quantification (LLOQ) of VX and ODV were 3.35 and 3.86 ng/mL, respectively. The intra- and inter-day coefficients of variation were within 15%.

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© 2009 by The Japan Society for Analytical Chemistry
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