Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Rapid Communications
Analysis of Protein-induced Calcium Carbonate Crystals in Soft Coral by Near-Field IR Microspectroscopy
M. Azizur RAHMANTamotsu OOMORI
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2009 Volume 25 Issue 2 Pages 153-155


The protein-induced chemical structure in calcified organisms is an important factor contributing to their growth, but until now no direct evidence has been reported. Hence, we demonstrate a new analytical technique that combines an FTIR spectrometer and an apertureless-type near-field microscope with high spatial resolution. The complete structural composition of the biomineralized structure of marine organisms has been measured using this technique. The results show that -OH is distributed over the center of the mineral phase of small calcite, which was formed from interactions of matrix proteins.

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© 2009 by The Japan Society for Analytical Chemistry
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