Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Selectivity on Ion Transport across Bilayer Lipid Membranes in the Presence of Gramicidin A
Shintaro KUBOTAShunsuke OZAKIJun ONISHIKenji KANOOsamu SHIRAI
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2009 Volume 25 Issue 2 Pages 189-193

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Abstract

Ion transport from one aqueous (W1) to another (W2) across bilayer lipid membranes (BLM) containing gramicidin A (GA) was investigated by recording current fluctuations, when various alkali metal chlorides and potassium salts were used as supporting electrolytes. The magnitude of the single-channel current at a given membrane potential depended on not only the cationic species, but also on the anionic species, and then it decreased with an increase in the diameter of the anion when the diameter of the anion was larger than the pore size of the GA channel. The baseline of the recording current, however, increased with an increase in the diameter of the anion, and its height depended on the concentration of GA in the BLM. The results indicate that GA serves as not only a channel-forming compound, but also as a carrier compound in the BLM.

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© 2009 by The Japan Society for Analytical Chemistry
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