Simultaneous RP-HPLC Determination of Sotalol, Metoprolol, α-Hydroxymetoprolol, Paracetamol and Its Glucuronide and Sulfate Metabolites in Human Urine

Abstract

The HPLC method for the determination of sotalol (SOT), metoprolol (MET) and α-hydroxymetoprolol metabolite (MET-H), paracetamol (PAR), paracetamol glucuronide (PAR-G) and paracetamol sulfate (PAR-S) in human urine is described. Analyses were carried out on a reversed-phase LiChroCART^® Purospher^® C18e column (125 mm×3 mm, 5 μm particles) (Merck) with gradient elution as well as spectrophotometric and fluorometric detection. Good resolution of the analyzed substances was obtained within a time range of no longer than 15 min. The linearity ranges of the callibration curves in human urine (as matrix) were: 3.25 − 45 μg ml⁻¹ (SOT), 0.75 − 40 μg ml⁻¹ (MET), 0.6 − 40 μg ml⁻¹ (MET-H), 4.6 − 60 μg ml⁻¹ (PAR-G), 4.95 − 50 μg ml⁻¹ (PAR-S), 1.95 − 45 μg ml⁻¹ (PAR). An application to human urine samples was performed.