Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
Original Papers
Headspace Solid-Phase Microextraction-Gas Chromatography Method for the Determination of Valproic Acid in Human Serum, and Formulations Using Hollow-Fiber Coated Wire
Mir Ali FARAJZADEHKhalil FARHADIAmir A. MATINParia HASHEMIAbolghasem JOUYBAN
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2009 Volume 25 Issue 7 Pages 875-879

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Abstract

A method was developed for the extraction of valproic acid (VPA) by hollow-fiber coated wire as a lab-made solid-phase microextraction (SPME) fiber and its determination by capillary gas chromatography in human serum and pharmaceutical formulations. In this study, a piece of copper wire coated by polypropylene hollow-fiber membrane was used as a SPME fiber, and its efficiency for the extraction of VPA from the headspace of samples prior to gas chromatographic analysis was evaluated. The optimum conditions of microextraction process were selected, and the limit of detection for VPA was found to be 85 μg L−1 in solution and 1.7 mg L−1 in human serum. A low detection limit, a wide linear dynamic range (0.25 − 100 mg L−1), good repeatability (RSD%<4 in formulations and RSD%<7 in serum samples) and a higher mechanical durability due to its metallic base are some of the most important advantages of the proposed fiber.

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© 2009 by The Japan Society for Analytical Chemistry
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