Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
Notes
In-Gel Staining of Proteins in Native Poly Acryl Amide Gel Electrophoresis Using Tetrakis(4-sulfonato phenyl)porphyrin
Kalivarathan DIVAKARVijayan SUJATHASridhar BARATHKrishnamurthy SRINATHPennathur GAUTAM
Author information
JOURNALS FREE ACCESS

2011 Volume 27 Issue 1 Pages 101-103

Details
Abstract

Protein identification in polyacrylamide gel electrophoresis (PAGE) requires post-electrophoretic steps like fixing, staining and destaining of the gel, which are time-consuming and cumbersome. We have developed a method for direct visualization of protein bands in PAGE using tetrakis(4-sulfonato phenyl)porphyrin (TPPS) as a dye without the need for any post electrophoretic steps, where separation and recovery of enzymes become much easier for further analysis. Activity staining was done to prove that the biochemical activity of the enzymes was preserved after electrophoresis.

Information related to the author
© 2011 by The Japan Society for Analytical Chemistry
Previous article Next article
feedback
Top