Facile and Effective Pretreatment Using Stop and Go Extraction Tips for LC-MS/MS Analysis of Trace Amounts of DNA Adducts

Hiroya MURAKAMI; Rieko KAWAMURA; Takayoshi SAKAKIBARA; Yukihiro ESAKA; Yasushi ISHIHAMA; Bunji UNO

doi:10.2116/analsci.30.519

Notes

Facile and Effective Pretreatment Using Stop and Go Extraction Tips for LC-MS/MS Analysis of Trace Amounts of DNA Adducts

Hiroya MURAKAMI, Rieko KAWAMURA, Takayoshi SAKAKIBARA, Yukihiro ESAKA, Yasushi ISHIHAMA, Bunji UNO

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Keywords: DNA adduct, stop and go extraction tip, LC-MS/MS analysis, poly(styrene-divinylbenzene) copolymer

JOURNAL FREE ACCESS

2014 Volume 30 Issue 4 Pages 519-522

DOI https://doi.org/10.2116/analsci.30.519

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Abstract

The preparation and application of a stop and go extraction tip (StageTip) used for pre-purification of sample solutions for LC-MS/MS analysis of DNA adducts was simplified and improved to increase throughput while maintaining high adduct selectivity. It was demonstrated that the StageTip composed of two sheets of a poly(styrene-divinylbenzene) copolymer disk could be easily prepared and proved useful for selective extraction of trace amounts of DNA adducts from a sample solution containing a great quantity of normal deoxynucleosides.

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