Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Rapid Communications
PNA-tagged Peptide Microarrays for Ratiometric Activity Detection of Cellular Protein Kinases
Hiromu IKEDAYoshihiro YAYAMAAkito HATAJumpei KAMIMOTOTatsuhiro YAMAMOTOTakeshi MORIYoshiki KATAYAMA
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2014 Volume 30 Issue 6 Pages 631-635

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Abstract

Peptide microarrays can be used to measure the activity of multiple protein kinases (PKs), which can be used to elucidate kinomics for drug discovery and diagnosis. Here we demonstrated a new microarray for ratiometric detection of the activity of PKs using peptide nucleic acid (PNA)-tagged peptides labeled with two different fluorophores, Cy3 and Cy5. We successfully detected cellular PK activities based on ratiometry, and applied the system for evaluation of an inhibitory drug.

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© 2014 by The Japan Society for Analytical Chemistry
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