Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
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Nanoporous Waveguide Spectroscopy for the Estimation of Enzyme Adsorption on Mesoporous Silica
Hiroyuki ARAFUNEKazuhiro HOTTATetsuji ITOHNorio TERAMAEAkira YAMAGUCHI
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2017 Volume 33 Issue 4 Pages 473-476

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Abstract

Mesoporous silica is considered as promising host material for enzymes due to its uniform pore size of enzyme dimensions and tunable surface chemical properties. In this study, we applied nanoporous waveguide (NPWG) spectroscopy to observe adsorption dynamics of heme proteins with different molecular size within mesoporous silica film modified with different surface functional groups. Since NPWG spectroscopy provides kinetic information and rough quantification of adsorption amount, it is useful to study the adsorption process of enzymes within inorganic nanoporous materials.

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© 2017 by The Japan Society for Analytical Chemistry
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