2018 Volume 34 Issue 11 Pages 1231-1236
A microchip electrophoresis (ME) device with off-channel contact conductivity detection (C2D) was constructed using top–bottom antiparallel indium tin oxide (ITO) electrodes and a cross-type microchannel. The 500-m wide top–bottom antiparallel decouplers were found to effectively decrease the interference of the electrophoretic current. The cross-type microchannel was formed by bonding the patterned negative photoresist microstructures and the two top–bottom opposed ITO-deposited glass substrates. Five seconds of 150 V/cm injection field and the 100 V/cm separation field equipped with the C2D of AC 200 mV excitation voltage provided adequate ME operational parameters to obtain the K+ and Na+ peaks separation. The ME devices obtained good coefficients in a range of 11000 μM for the K+ and Na+ detection. The calculated limit of detection was 1 μM. This design for off-channel and top–bottom antiparallel electrodes shows that C2D ME devices have great potential for the measurement of inorganic ions.