Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Extracellular Bio-imaging of Acetylcholine-stimulated PC12 Cells Using a Calcium and Potassium Multi-ion Image Sensor
Sota MATSUBARyo KATOKoichi OKUMURAKazuaki SAWADAToshiaki HATTORI
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2018 Volume 34 Issue 5 Pages 553-558

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Abstract

In biochemistry, Ca2+ and K+ play essential roles to control signal transduction. Much interest has been focused on ion-imaging, which facilitates understanding of their ion flux dynamics. In this paper, we report a calcium and potassium multi-ion image sensor and its application to living cells (PC12). The multi-ion sensor had two selective plasticized poly(vinyl chloride) membranes containing ionophores. Each region on the sensor responded to only the corresponding ion. The multi-ion sensor has many advantages including not only label-free and real-time measurement but also simultaneous detection of Ca2+ and K+. Cultured PC12 cells treated with nerve growth factor were prepared, and a practical observation for the cells was conducted with the sensor. After the PC12 cells were stimulated by acetylcholine, only the extracellular Ca2+ concentration increased while there was no increase in the extracellular K+ concentration. Through the practical observation, we demonstrated that the sensor was helpful for analyzing the cell events with changing Ca2+ and/or K+ concentration.

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© 2018 by The Japan Society for Analytical Chemistry
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