Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
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Sensitive Analysis of Sialic Acid and Related Compound by Hydrophilic Interaction Liquid Chromatography Using Fluorescence Detection after Derivatization with DBD-PZ
Akane KAWASAKIMakoto YASUDAKen-ichi MAWATARITomoko FUKUUCHINoriko YAMAOKAKiyoko KANEKORyosuke IIJIMASatoru YUIMotonobu SATOHKazuya NAKAGOMI
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2018 Volume 34 Issue 7 Pages 841-844

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Abstract

N-Acetylneuraminic acid (NANA) has been reported to react with hydrogen peroxide in vitro to produce 4-(acetylamino)-2,4-dideoxy-D-glycero-D-galacto-octonic acid (ADOA). We labeled NANA and ADOA with 4-(N,N-dimethylaminosulfonyl)-7-piperazino-2,1,3-benzoxadiazole (DBD-PZ) for simultaneous detection. The derivatized NANA and ADOA were separated using hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection. The calibration curves of DBD-PZ-derivatized NANA and ADOA showed good linearity in the range of 221 fmol to 1.5 nmol, and 44 fmol to 1.5 nmol, respectively. This analytical method has high specificity and is useful for the detection of NANA and ADOA in saliva and serum.

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© 2018 by The Japan Society for Analytical Chemistry
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