Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
An Ultrasensitive Colorimetric Strategy for Detection of Cadmium Based on the Peroxidase-like Activity of G-Quadruplex-Cd(II) Specific Aptamer
Bin ZHOUYa-Ting CHENXin-Yi YANGYong-Sheng WANGXi-Jiang HUQing-Li SUO
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2019 Volume 35 Issue 3 Pages 277-282


We rationally designed an ultrasensitive and label-free sensing platform for determination of cadmium (Cd). The sensing platform contains G-quadruplex-Cd(II) specific aptamer (GCDSA) constructed by incorporating G-rich sequence at the end of 5′ and the critical domain of the Cd-4 aptamer. GCDSA designed act as both a special recognition sequence for Cd2+ and a signal DNAzyme. In absence of Cd2+, GCDSA may mainly exist in a random coil sequence. Upon addition of Cd2+, GCDSA could probably be induced to fold into a G-quadruplex structure. The generation of plentiful active G-quadruplex interacts with hemin to form a peroxidase-like DNAzyme, leading to increased absorbance signal of the sensing system. ΔA was directly proportional to the two segments of concentrations for Cd2+, with the detection of limit of 0.15 nM. The proposed method avoids the labeled oligonucleotides and allows directly quantitative analysis of the samples by cheap instruments, with an excellent dynamic range.

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© 2019 by The Japan Society for Analytical Chemistry
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