Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Detection of Gold Nanoparticles Aggregation Using Light Scattering for Molecular Sensing
Yuki YANOMasamichi NISOUGIYuki YANO-OZAWATsuyoshi OHGUNIAtsushi OGAWAMizuo MAEDATsuyoshi ASAHITamotsu ZAKO
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2019 Volume 35 Issue 6 Pages 685-690


Gold nanoparticles (AuNPs) have been commonly used in molecular sensing, in the form of observation of the color change from red to blue of the AuNP solution, caused by target-molecule-induced AuNP aggregation. In this work, the changes in absorbance and scattering spectra caused by AuNP aggregation were studied using thrombin-induced AuNP aggregation as a model. We demonstrated for the first time that scattering spectra is more sensitive to the changes owing to AuNP aggregation than absorbance spectra. Moreover, a digital color analysis of darkfield images using dark field microscopy (DFM) facilitated a simple method for detection of AuNPs aggregation without the use of spectroscopic analysis. Furthermore, we demonstrated that DFM is useful for detecting AuNPs aggregation in a colored solution, in which the color change by AuNPs aggregation is not visible.

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© 2019 by The Japan Society for Analytical Chemistry
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