Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Enhanced Mimetic Enzyme Activity of Phosphorylated Porphyrin Nanocomposite Induced by Localized Surface Plasmon Resonance for Colorimetric Assay
Yan YANGFang TANXiaoxue XIEXiumei YANGZaichun ZHOUKeqin DENGHaowen HUANG
Author information

2019 Volume 35 Issue 6 Pages 691-699


Plasmon-enhanced light harvesting has been of great interest to enhance the catalytic efficiency of some composites or hybrids. The enhanced peroxidase-like activity of phosphorylated iron(III) porphyrin (TPPFe(III))-based nanocomposite, induced by localized surface plasmon resonance for a colorimetric assay, was developed in this study. Firstly, a phosphate group modification strategy was adopted to synthesize water-soluble iron(III) porphyrin materials. Then, the as-synthesized TPPFe(III) was covalently attached to core-shell gold nanorods (GNRs), GNR@Au2S/AuAgS, to form TPPFe(III)-GNR@Au2S/AuAgS nanocomposite, which shows greatly enhanced peroxidase-like activity compared to TPPFe(III). A mechanism for the enhanced peroxidase-like activity of TPPFe(III)-GNR@Au2S/AuAgS was proposed, which results from a synergic effect of hot electrons excited by localized surface plasmon resonance and photogenerated electrons of the TPPFe(III), verified by experiments. Furthermore, a fast colorimetric assay for the detection of H2O2 and glucose was established based on the unique property of TPPFe(III)-GNR@Au2S/AuAgS. This colorimetric assay was applied to determine practical human serum samples; satisfactory results demonstrate this method has high accuracy. The present study would not only provide some insights into the mechanism of plasmon-activated enzyme-like reactions, but also offer new strategies for improving the catalytic activity of a mimetic enzyme.

Fullsize Image
Information related to the author
© 2019 by The Japan Society for Analytical Chemistry
Previous article Next article