Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Comparative Study of Electrochemical Sensors Based on Enzyme Immobilized into Polyelectrolyte Microcapsules and into Chitosan Gel
Yulia V. PLEKHANOVASergei A. TIKHONENKOAlexey V. DUBROVSKYAlexander L. KIMEgor V. MUSINGou-Jen WANGIren E. KUZNETSOVAVladimir V. KOLESOVAnatoly N. RESHETILOV
Author information
JOURNAL FREE ACCESS

2019 Volume 35 Issue 9 Pages 1037-1043

Details
Abstract

The characteristics of an electrochemical biosensor based on a Prussian-blue screen-printed electrode containing glucose oxidase incorporated into polyelectrolyte microcapsules (PMC) are considered. PMC with the embedded enzyme were formed using sodium polystyrene sulfonate and poly(allylamine hydrochloride). The characteristics were compared with those of the enzyme immobilized in chitosan gel. We assessed the dependences of biosensor signals on the composition of the buffer solution, on the glucose concentration; the operational and long-term stabilities. The enzyme immobilized in PMC proved to be more sensitive to buffer molarity at a maximum within 35 – 40 mM. The apparent Michaelis constants were 1.5 and 4.1 mM at the immobilization in, respectively, chitosan and PMC. The developed biosensors were used to assay commercial juices. The biosensors’ data on the glucose contents were shown to have a high correlation with the standard spectrophotometric assay (0.92 – 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis.

Fullsize Image
Information related to the author
© 2019 by The Japan Society for Analytical Chemistry
Previous article Next article
feedback
Top