1985 Volume 1 Issue 1 Pages 73-76
A simple and rapid spectrophotometric method for the determination of penicillins has been developed. The method is based on the measurement of ultraviolet absorbance at 323-346nm of reaction products with 1-2M 1, 2, 4-triazole and|1×10−3M mercury(II) chloride in solution (pH 9.0) for 10min at 60°C. The method, which permits detection of concentrations of penicillins down to 0.1μg/ml, is accurate and precise. The assay of amino-penicillins was interfered with the coexistence of the corresponding penicilloic acids, while the method is specific to other intact penicillins without interference of the corresponding penicilloic acids.
