Gas Chromatographic Determination of Uric Acid in Serum with Uricase-Catalase System
1985 年 1 巻 4 号 p. 377-379
詳細
1985 年 1 巻 4 号 p. 377-379
A new method for the gas chromatographic determination of uric acid in serum using uricase-catalase system is described. This method is based on the combination of the enzymatic conversion of methanol into formaldehyde through the action of hydrogen peroxide formed from uric acid with uricase and derivatization of the resulting formaldehyde with pentafluorobenzyloxylamine. Suitable conditions for the use of uricase and influence of presence of co-existing compounds were investigated. The method is superior to the colorimetric method with respect to specificity and sensitivity. Linear calibration graphs passing through the origin were obtained in the range 8-200μg ml-1 uric acid in serum for FID and 1-12μg ml-1 for ECD using 0.1ml of serum.
