1994 Volume 10 Issue 6 Pages 919-922
A continuous flow chemiluminometric method for determining 10-8 - 10-5M of folic acid in pharmaceutical formulations is described. The method is based on the chemiluminescence (CL) produced by the action of cerium(IV) on folic acid in an acidic medium. The emission intensity is greatly enhanced when Rhodamine B is used as a sensitizer. The procedure is automated, requires no sample pretreatment and solutions can be analyzed at a rate of 180 samples h-1with a relative standard deviation of 0.54% at 1.1×10-5M folic acid (n=10).