A Continuous Flow System for Sensitive Urushiol Detection Using a New Chemiluminescent Reaction

Abstract

By using a new chemiluminescent (CL) reaction system consisting of an urushiol/hydrogen peroxide/sodium hydroxide/ dye-sensitizer, a sensitive urushiol detection system was developed which featured a continuous flow method. The system comprises three-channel flow manifolds including an introduction manifold for the urushiol sample. The system has some advantages as regards instrumentation, sensitivity and analytical time; the detection limit is 1.O×10^-5M (S/N=2); the analytical time required is less than 30s for one sample feeding; the relative standard deviation is 6.2% for 5 repeated runs of the 1.0×10^-4M urushiol solution. A possible explanation for the CL reaction mechanism is also presented.