1995 Volume 11 Issue 3 Pages 385-388
Free fatty acids (FFAs) were determined by supercritical-fluid chromatography (SFC) on an inert ODS silica-gel column using carbon dioxide as a mobile phase without a modifier. The standard samples of FFAs with carbon numbers of 6 to 26 (even numbers) were completely separated using the pressure-programming mode. The retention behavior of FFAs was investigated in terms of the density of the mobile phase, and the FFAs were separated according to a reversed-phase mode in liquid-chromatographic terms. The composition of fatty acids of coconut oil was determined by the SFC, and was compared with gas chromatography. A good agreement was obtained. The chromatographic separation of FFAs obtained from fish oil as well as the enzymatic hydrolysis products of the fish oil were also conducted.
