In vivo ¹¹B NMR Observation of Plant Tissue

Abstract

In vivo ¹¹B NMR spectra of plant tissue, namely, radish roots, apple fruit, cabbage leaves and komatsuna plant leaves and roots, were successfully obtained. In the spectra, the signals of borate monoester and diester, together with boric acid, were observed, which shows that the in vivo ¹¹B NMR technique provides direct evidence that the borate esters exist in plant tissue. The spectra of juice and residue prepared from radish roots and apple fruit revealed that the signals, especially those of borate esters, in the intact plant spectra were derived from the water-soluble fraction in the plant cell.