Interaction of Corticosterone Antibody and Glutaraldehyde Adsorbed on Pt Electrodes Modified with Silane

Abstract

The interaction of a corticosterone antibody and glutaraldehyde adsorbed on a Pt electrode modified with silane was examined by cyclic voltammetry. A mirror-polished Pt wire was oxidized for 10min at +1.3V vs. Ag/AgCl in 1.0M H₂SO₄. The oxidized Pt wire was coated with 3-aminopropyltrimethoxysilane under N₂ gas for 1h at room temperature in benzene, and then modified with glutaraldehyde. The modified electrodes were tested for their electrocatalytic properties and interaction with the corticosterone antibody. The catholic peak currents (-0.4V) exhibited a reproducible linearity of the current intensity vs. the concentration of anticorticosterone.