Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Analysis of Pyrene Fluorescence Emission by Fast Fourier Transformation
Masayuki ITAGAKIMasaki HOSONOKunihiro WATANABE
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1997 Volume 13 Issue 6 Pages 991-996

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Abstract

The transfer function, H(ω), for fluorescence emission of pyrene in ethanol has been calculated from the excitation ray and fluorescence time profiles by the fast Fourier transformation (FFT) process. The H(ω) describes a clear semicircle on the Nyquist plane at low pyrene concentration. On the other hand, at high pyrene concentration the real part of H(ω) takes a negative value and the locus deviates from a semicircle at high frequency region. The theoretical expressions of the H(ω) have been derived on the basis of the fluorescence emission model containing the formation of excimer: H(ω)=2.3αεlcP{kf1(+K2)+kf2kecP}/{(+K1)(+K2)-K3} K1=kq1+kf1+kecP, K2=kb+kf2+kq2, K3=kbkecP where α is correction factor, ε is the molar extinction coefficient, l is the cell length and cP is the concentration of pyrene. Ke and kb†are the formation rate constant and its reverse rate constant, respectively. kf1 and kq1 represent rate constants of fluorescence emission and quenching of excited pyrene, respectively. kf2 and kq2 represent rate constants of fluorescence emission and quenching of excimer, respectively. The results simulated by this equation were in good agreement with experimental results. In the course of simulation, kinetic parameters for the pyrene fluorescence were obtained.

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