Analysis of Calmodulin Fragments and Preparation of the Peptides Containing Calcium Binding Domains by High Performance Liquid Chromatography

Abstract

The degradation process of calmodulin with trypsin in the presence or absence of calcium ions was reinvestigated by the analysis on HPLC. All tryptic peptides and intermediate peptides were mapped on this system. On the basis of these results, the strategy of the preparation of various calmodulin fragments was devised. Twelve peptides which contain calcium binding sites were obtained.