Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Amperometric Biosensors Based on a Biocatalyst Electrode with Entrapped Mediator
Mitsugi SENDATokuji IKEDAKojiro MIKIHiroshi HIASA
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1986 Volume 2 Issue 6 Pages 501-506


A mathematical model of a biocatalyst electrode with entrapped mediator is presented. The electrode is a benzoquinone (BQ)-mixed carbon paste electrode (CPE), on which glucose oxidase (GOD) is immobilized by coating with a polymer film or semipermeable membrane (s.m, a film-coated GOD-BQ-CPE). BQ is dissolved in the immobilized-enzyme layer(e.l.) to mediate the electron transfer between the electrode and the enzyme. The model is focused on the diffusion accompanied with enzyme reaction of substrate (D-glucose) and mediators (BQ and its reduced form) in the e.l. and the diffusion in the sm. The amperometric response of the electrode is analyzed as a function of transport, kinetic, and geometrical parameters and compared with experimental results. A glucose sensor based on a film-coated GOD-BQ-CPE has a wide concentration range of response and is not affected by oxygen in test solution. Biosensors for D-gluconate and ethanol based on film-coated gluconate dehydrogenase-BQ- and alcohol dehydrogenase-NAD-CPE′s, respectively, are presented.

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