1987 Volume 3 Issue 2 Pages 171-174
An anion-exchange resin, Amberlite IRA 900 modified with manganese-tetrakis(sulfophenyl)porphine (MnTPPSr), was used in place of peroxidase for the determination of hydrogen peroxide by the following enzyme-re-action: 2H2O2+phenol+4-aminoantipyrine →<peroxidase> quinoid dye(λmpx=505nm)+4H2O. The calibration curve was linear in the range from 5μg to 50μg of hydrogen peroxide with a relative standard deviation of 1.05% (n=10).The absorbance of the quinoid dye formed by the use of Mn-TPPSr reached approximately 80% of that obtained by the control peroxidase method. The activity of MnTPPSr did not deteriorate over repeated uses.Only slight interferences of foreign substances were found except for the case of ascorbic acid.