Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Sequence-Selective Separation of Oligonucleotides and DNA Fragments by Using Polyethyleneglycol-Bound Intercalators
Shigeori TAKENAKAKunihiko DOHTSUNaotoshi NAKASHIMAMakoto TAKAGI
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1987 Volume 3 Issue 6 Pages 557-560

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Abstract

Anthracene; acridine and quinacrine which possess base- and sequence-selectivity in their DNA-binding (intercalating) properties were covalently bound to poly(ethyleneglycol) (PEG) via ester bonds. These PEG-bound intercalators induced the retardation of DNA restriction fragments and oligonucleotides (pentadecamers) in the electrophoresis in agarose gel and polyacrylamide gel, respectively. The pattern of mobility retardation depended on the base composition of the DNA fragments and oligonucleotides, reflecting the base selectivity of the parent intercalators. The PEG-bound intercalators also discriminated the base sequence, and the oligonucleotides of nearly sequence-isomereric structures such as (TA)7T and A8T7 were mutually readily separated.

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