Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Fluorometric Determination of Kynurenic Acid in Human Serum by High Performance Liquid Chromatography Coupled with Postcolumn Photochemical Reaction with Hydrogen Peroxide
Ken-ichi MAWATARIFumio IINUMAMitsuo WATANABE
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1988 Volume 4 Issue 2 Pages 195-197

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Abstract

A high performance liquid chromatographic method involving postcolumn photochemical reaction and fluorometric detection has been developed for the determination of kynurenic acid. Kynurenic acid was separated on a reversed phase column with a mobile phase containing hydrogen peroxide and irradiated by ultraviolet light to give fluorescence. The method allows the determination of kynurenic acid in human serum at concentrations as low as 0.05pmol/100μl.

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