Fluorometric Determination of Kynurenic Acid in Human Serum by High Performance Liquid Chromatography Coupled with Postcolumn Photochemical Reaction with Hydrogen Peroxide

Ken-ichi MAWATARI; Fumio IINUMA; Mitsuo WATANABE

doi:10.2116/analsci.4.195

Ken-ichi MAWATARI, Fumio IINUMA, Mitsuo WATANABE

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Keywords: Kynurenic acid, postcolumn photochemical reaction, hydrogen peroxide, tryptophan metabolite, fluorescence, high performance liquid chromatography

JOURNAL FREE ACCESS

1988 Volume 4 Issue 2 Pages 195-197

DOI https://doi.org/10.2116/analsci.4.195

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Abstract

A high performance liquid chromatographic method involving postcolumn photochemical reaction and fluorometric detection has been developed for the determination of kynurenic acid. Kynurenic acid was separated on a reversed phase column with a mobile phase containing hydrogen peroxide and irradiated by ultraviolet light to give fluorescence. The method allows the determination of kynurenic acid in human serum at concentrations as low as 0.05pmol/100μl.

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