Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
DETERMINATION OF MONOPHOSPHATE-TYPE NUCLEOTIDES BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH PHOSPHORUS-SELECTIVE DETECTION
Wenzhi HuToyohide TakeuchiHiroki Haraguchi
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1991 Volume 7 Issue Supple Pages 135-138

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Abstract

A selective, method for the determination of the determination of monophosphate-type nucleotides based on the molybdenum blue method has been developed. The monophosphates were decomposed to produce the orthophosphate-molybdenum blue complex by heating at 140°C without any oxidizing agent, while di- or triphosphates hardly produced the complex under the same condition. The separation of monophosphates was carried out in the ion-exchange mode, and the detection limits were 25ppb as P for the monophosphates examined.

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© The Japan Society for Analytical Chemistry
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