DETERMINATION OF ZINC(II) CHLOROPHYLLS AND THEIR DERIVATIVES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUOROMETRIC DETECTION

Abstract

An HPLC method with fluorometric detection was developed for the determination of zinc(II) chlorophyll a and b ( Zn-chl-a, b) and zinc(II) chlorophyll ins (Zn-chlns). The analytical values by the HPLC method with UV and fluorometric detection were compared with those obtained by the spectrophotometric method. The values obtained by the HPLC method using a fluorometric detector were the most reproducible of three analytical methods. It was confirmed that the interferences from the absorption of coexistent pigments are removed by separation using HPLC and the overlapping of peaks from other metallochlorophylls is avoided by using a fluorescence spectromonitor.