1991 Volume 7 Issue Supple Pages 157-160
Anion-Exchange Chromatography followed by pulsed amperometric detection (AE-PAD) was applied to the analysis of the monosaccharides in lipopolysaccharides (LPS). Under alkaline condition, nine underivatized monosaccharides (fucose, rhamnose, galactosamine, arabinose, glucosamine, galactose, glucose, xylose and mannose) were separated within 25 minutes at an eluent flowrate of 1.0ml/min. Based on this method, the LPS samples, which were extracted from Salmonella abortus equi, were analyzed. The results revealed the monosaccharide constituents in LPS samples.