Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
ANALYSIS OF MONOSACCHARIDES IN LIPOPOLYSACCHARIDES BY ANION-EXCHANGE CHROMATOGRAPHY WITH PULSED AMPEROMETRIC DETECTION
QUN SUNSHIFEN MOUJIHONG LINDEPEI LU
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1991 Volume 7 Issue Supple Pages 157-160

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Abstract

Anion-Exchange Chromatography followed by pulsed amperometric detection (AE-PAD) was applied to the analysis of the monosaccharides in lipopolysaccharides (LPS). Under alkaline condition, nine underivatized monosaccharides (fucose, rhamnose, galactosamine, arabinose, glucosamine, galactose, glucose, xylose and mannose) were separated within 25 minutes at an eluent flowrate of 1.0ml/min. Based on this method, the LPS samples, which were extracted from Salmonella abortus equi, were analyzed. The results revealed the monosaccharide constituents in LPS samples.

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© The Japan Society for Analytical Chemistry
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