SEPARATION CHARACTERISTICS OF WHALE MYOGLOBIN BY CHROMATOFOCUSING AND SIZE EXCLUSION CHROMATOGRAPHY WITH AID OF A PHOTODIODE ARRAY DETECTOR

Abstract

Separation of sperm whale myoglobin(Mb) was studied by chromatofocusing(CF) and size exclusion chromatography(SEC). At least 9 peaks were observed in the CF chromatogram. This suggests the existence of 9 different components with different isoelectric points. On the other hand, all these components gave the same elution time with each other in SEC, when each peak fraction separated by CF was injected. Thus the separation characteristics in CF was investigated with aid of a photodiode array detector to elucidate the separated components.