Photographic Detection of Sub-Picomole Fluorescers by Peroxyoxalate Chemiluminescence Reaction

Abstract

Femtomole fluorescers such as perylene or 5, 12-bis(phenylethynyl)naphthacence (PEN) were detected photographicallythrough the peroxyoxalate chemiluminescence reaction (CL). With the use of X-ray film, the CL emissions from 9.6× 10^-14mol fluorescers were recorded as black-and-white spots, while they are recorded as color spots on color films such as instant color films (ISO 80-100) or high speed 35mm roll films (ISO 400 and 3200) with the detection limit of 9.6× 10^-13mol. This technique has been applied to the detection of albumin labeled with fluorescer. As little as 0.30μg of pyrene labeled bovine serum albumin or 2.4μg of tetramethylrhodamine labeled albumin was detected by this technique.