High-Performance Liquid Chromatographic Separation of Histidine Oligopeptides

Jun-ichi UEDA; Akira HANAKI; Tadashi YASUHARA; Terumi NAKAJIMA; Shosuke SOFUKU

doi:10.2116/analsci.8.487

Jun-ichi UEDA, Akira HANAKI, Tadashi YASUHARA, Terumi NAKAJIMA, Shosuke SOFUKU

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Keywords: High-performance liquid chromatography, histidine peptides, cation-exchanger, silica gel

JOURNAL FREE ACCESS

1992 Volume 8 Issue 4 Pages 487-489

DOI https://doi.org/10.2116/analsci.8.487

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Abstract

Histidine oligopeptides which differ by one histidyl residue in length up to a total of 20 amino acid residues were separated by high-performance liquid chromatography on a commercially available strong cation-exchange column (TSK gel SP- 25W) using isocratic elution with a NaCl solution.

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