1993 Volume 9 Issue 5 Pages 625-629
Protochlorophylls of inner seed coats of pumpkin were analyzed by three-dimensional reversed phase high-performance liquid chromatography. Three types of precursors, 2, 4-divinyl-protochlorophyll a, 2, 4-divinyl-protopheophytin a and 2-vinyl-protopheophytin a, were identified. Each could be subdivided into three species based on the variety of the esterifying alcohol moiety, namely tetrahydrogeranyl geraniol, dihydrogeranyl geraniol or geranyl geraniol. The phytol ester of divinyl-protopheophytin a was also detected. These precursors were simultaneously identified on the three-dimensional chromatogram.