1993 Volume 9 Issue 6 Pages 803-806
A liquid-chromatographic (LC) on-column derivatization method coupled with a column-switching technique has been developed for the determination of histamine (Him) and 1-methylhistamine (MH) in biological materials. This method was designed to involve both a sample purification step and a fluorescence derivatization step for the direct application of a deproteinized sample to the LC system. A crude extract of mouse tissue injected onto the LC system was purified on an ion-exchange polymer column, and then Him and MH were derivatized to each fluorophore, followed by chromatography on a reversed-phase C18 polymer column. Optimization of the LC conditions has led to the development of a simple analytical method for the simultaneous determination of Him and MH with good reproducibility and a wide linear range of 0.01-10μg ml-1. Application of this method to the determination of Him and MH in mouse tissues and fermented soy foods resulted in overall mean recoveries greater than 93% at fortification values of 1-100μg g-1
