2018 Volume 71 Issue 5 Pages 225-231
Tosoh TRC Ready MP is a newly developed automatic Mycoplasma pneumoniae rapid detection method using a transcription reverse-transcription concerted reaction. We analyzed the performance of the TRCReady MP assay by comparing with polymerase chain reaction (PCR) method and Loop mediated isothermal amplification (LAMP) method with clinical samples. As for the result of 290 clinical samples in which detection of M. pneumoniae was targeted, the rate of concordance between TRCReady MP and PCR method was 96.2% (sensitivity, 98.2%; specificity, 95.7%), and that between TRCReady and LAMP method was 96.2% (sensitivity, 100%; specificity, 95.3%). It takes within one hour for detection using TRCReady MP after sample preparation processing. These results indicate that TRCReady MP is useful for the rapid diagnosis of M. pneumoniae infection.
