STUDIES ON BIOSYNTHESIS OF KASUGAMYCIN. V BIOSYNTHESIS OF THE AMIDINE GROUP

Abstract

During production of kasugamycin, ¹⁵N-glycine was added, yielding ¹⁵-Nkasugamycin. ¹⁵N-Kasugamycin was treated with baryta, yielding ammonia f rom the carboxyformidoyl group (the side chain). The two amino groups of kasuganobiosamine which was obtained by baryta treatment were degraded to ammonia. Mass spectroscopic analysis of ammonia from these two sources showed that the nitrogen atom of glycine is incorporated into the imino nitrogen of the carboxyformidoyl group.