STUDIES ON THE MODE OF ACTION OF ALTHIOMYCIN

Abstract

Among syntheses of DNA, RNA and protein in intact cells of E. coli B, althiomycin exhibited the strongest inhibition against protein synthesis. In the cell-free system althiomycin was confirmed to inhibit protein synthesis. On the other hand, it did not inhibit protein synthesis of reticulocyte either in the intact cells or in the cell-free system. By further studies in E. coli B systems the antibiotic was shown to inhibit the puromycin reaction, without inhibiting aminoacyl-tRNA synthesis and binding of aminoacyl-tRNA to ribosomes.