The Journal of Antibiotics
Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820
STUDIES ON VALIDAMYCINS, NEW ANTIBIOTICS. III
BIOASSAY METHODS FOR THE DETERMINATION OF VALIDAMYCIN
TAKASHI IWASAEIJI HIGASHIDEMOTOO SHIBATA
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1971 Volume 24 Issue 2 Pages 114-118

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Abstract

Validamycin was found to cause an abnormal branching at the tips of the hyphae of Pellicularia sasakii under specific conditions. By application of this phenomenon, an agar dilution method, "dendroid-test method", was established. In another method called the "reversed layer method", agar plates inoculated with P. sasakii are incubated for 40 to 45 hours, then covered with more agar medium and paper disks dipped in the sample solution are applied on the surface. After overnight incubation the diameters of the inhibition zones are measured.

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© Japan Antibiotics Research Association
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